What is Photomicrography?

Feature by Samyukta Mallick

Photomicrography is when one takes a photograph through a microscope.

Using X-Ray microscopes, precise pictures of living organisms can be clicked.

An aspect of capturing a beautiful micrograph is that the subject of the picture is tiny but you have to expand it in order for it to be seen.

All images in this feature are from Nikonsmallworld.com

Copper Crystals. Image Credit: Nikonsmallworld.com


There are some techniques that can be used when clicking a micrograph.


The main ones are listed below.

Confocal Imaging requires scanning the subject of the micrograph to make a digital copy of it. It also reduces the thickness of the specimen.

Starfish photographed using confocal microscopy, 2015 7th prize winner. Image Credit: nikonsmallworld.com

Brightfield Imaging is the easiest technique of microscopy. It is when the organism is simply
illuminated by a light and then taken a picture of.

Cross section of superconducting magnet wire etched with nitric acid, William Love, 3rd Place, 1984.


Darkfield Technique is when the light is illuminating the border and outside of the subject instead of going through it.

Sonchus asper (spiny sowthistle) flower stem section by Gerd Günther

Image Stacking is when multiple pictures taken from different angles are compiled and put together to form a result. The final micrograph has a much better depth and detailing. Image stacking is also used in normal photography.

Agatized dinosaur bone by Randy Fullbright


Stereo-micrography uses microscopes with two eye- pieces – stereomicroscopies. These are used to make the micrograph three-dimensional. These are specialised microscopes.

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